P118. Persistence of human papillomavirus infection after LEEP for High-Grade Squamous Intraepithelial Lesions in an Oncology Hospital of Ecuador.

Leopoldo Tinoco (EC), Ruth Jimbo (EC), Mónica Gracía (EC), Violeta Tinoco (EC), Sonia Carrillo (EC), René Masaquiza (EC), Beatriz Luna (EC)

[Tinoco] Hospital oncológico SOLCA- Quito-Ecuador- Universidad Central de Ecuador, [Jimbo] Universidad Católica del Ecuador(Quito-Ecuador), [Gracía] Universidad Católica del Ecuador-Quito- Ecuador, [Tinoco] Hospital Rodriguez Zambrano- (Manta-Ecuador), [Carrillo] GINECOMAST (Quito-Ecuador), [Masaquiza] Hospital oncológico SOLCA- Quito-Ecuador, [Luna] Hospital oncológico SOLCA- Quito-Ecuador

BACKGROUND: The persistence of HPV infection after loop electrosurgical excision procedure (LEEP) is a poorly studied and of unknown frequency in patients diagnosed with high-grade intraepithelial lesions. OBJECTIVE: To determine the persistence of HPV infection after LEEP in patients diagnosed High-Grade Squamous Intraepithelial Lesions METHODS: A prospective study involving 416 patients diagnosed with High-Grade Squamous Intraepithelial Lesions treated by LEEP between 2002 and 2017 was carried out. HPV, cytological and colposcopy tests were performed between 6 and 12 months after treatment for a period of no less than 5 years. In patients with a positive HPV result a new screening was performed at 6 months by means of hybrid capture to determine the persistence of infection. RESULTS: The mean age of patients included in the study was 45.1 years, 193 patients (46%) had cervical intraepithelial neoplasia (CIN) II and 223 (54%) presented CIN III by histological diagnosis. It was found that 65% (N: 272) had a negative result for HPV and 35% (N: 144) had a positive HPV infection, the patients with positive result the most frequent serotype was 16 (48%), followed by other serotypes (44%) and serotype 18 (8%). A 6-month follow-up of 57 patients with a positive result for HPV obtained a negative result in 36 patients (63%) and a positive result in 21 patients (37%), the distribution of HPV serotypes corresponded to other HPV serotypes (43%), serotype 16 (38%) and serotype 18 (18%). CONCLUSIONS: The persistence of HPV in patients with High-Grade Squamous Intraepithelial Lesions after underwent a LEEP was up to 37%, so a close follow-up of this group of patients is fundamental because the persistence of HPV constitutes a risk factor for the development of cervical cancer.